LNCAP（人前列腺癌肿瘤细胞）
CBP60346
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| I. General information | |
| Synonyms: | LNCAP |
| Background: | established from the left supraclavicular lymph node metastasis from a 50-year-old man with prostate carcinoma in 1977; cells were described in the literature to be androgen-sensitive |
| Species: | human (Homo sapiens) |
| Tissue: | prostate |
| Disease: | prostate carcinoma |
| Gender: | N/A |
| Morphology: | adherent fibroblastoid cells growing in aggregates and as single cells; |
| Growth Mode: | N/A |
| Doubling Time: | about 60 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+15%FBS LNCAP完全培养基，# CBP60346M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, MDH |
| Cytogenetics: | human hypotetraploid karyotype with 6% polyploidy - 88(84-93)<4n>XXYY, -2, -4, -6, -6, -13, -13, +2mar, t(1;15)(p22.3;q22.3)x2, del(2)(p16p23), del(10)(q24)x2, der(16)t(6;16)(p22.1;q22)x2 - del(10)(q24) is recurrent in prostate carcinoma - corresponds to published karyotype |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
