23132/87（人胃癌细胞）
CBP60475
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| I. General information | |
| Synonyms: | 23132/87 |
| Background: | established from the primary tumor of a 72-year-old man with gastric adenocarcinoma in 1987 |
| Species: | human (Homo sapiens) |
| Tissue: | gastric |
| Disease: | gastric adenocarcinoma |
| Gender: | N/A |
| Morphology: | adherent epithelial cells growing as confluent monolayers; |
| Growth Mode: | N/A |
| Doubling Time: | about 50 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI1640+10%FBS 23132/87完全培养基，# CBP60475M |
| Cryopreservation medium: |
90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -; (initial data published by the originators showed negativity, we found the cells to express cytokeratin-7) |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, MDH |
| Cytogenetics: | human hypertetraploid karyotype with 12% polyploidy - 47(45-52)<2n>XY, +20, t(1;15)(p11;p11), t(6;12) (p21;q21), i(13q), t(13;14)(p10;q10) - closely resembles published karyotype |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
