SiHa（人子宫鳞状癌细胞）
CBP60552
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| I. General information | |
| Synonyms: | SiHa |
| Background: | This line was established from fragments of a primary tissue sample obtained after surgery from a Japanese patient. |
| Species: | Homo sapiens, human |
| Tissue: | cervix |
| Disease: | grade II,squamous cell carcinoma |
| Gender: | Asian,female,55 years adult |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X CSF1PO: 12 D13S317: 11 D16S539: 12 D5S818: 9 D7S820: 10 THO1: 6,9 TPOX: 8 vWA: 14,17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM+10%FBS SiHa完全培养基，# CBP60552M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Oncogene: | p53 +; pRB + |
| Genes Expressed: | N/A |
| Tumor Formation: | Yes, in nude mice; forms poorly differentiated epidermoid carcinoma (grade III) |
| Comments: | Electron microscopic observations revealed presence of typical desmosomes at the cell junctions and an abundance of tonofilaments in the cytoplasm. Mycoplasma contamination was detected and eliminated in 1975.The line is reported to contain an integrated human papillomavirus type 16 genome (HPV-16, 1 to 2 copies per cell). For more information, please contact Cobioer (4008-750-250). |
