BHT-101（人甲状腺癌细胞）
CBP60706
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| I. General information | |
| Synonyms: | BHT-101 |
| Background: | established from the lymph node metastasis of a 63-year-old woman with anaplastic papillary thyroid carinoma; cells were described in the literature to not produce hormones, but to be partly positive for thyroglobulin- and thyroxine (T4) |
| Species: | human (Homo sapiens) |
| Tissue: | thyroid |
| Disease: | thyroid carcinoma |
| Gender: | N/A |
| Morphology: | epithelial-like cells growing in monolayers; |
| Growth Mode: | N/A |
| Doubling Time: | about 2-3 days |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM+20%FBS BHT-101完全培养基，# CBP60706M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP +, neurofilament -, thyroglobulin -, vimentin + |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, G6PD, LDH, NP |
| Cytogenetics: | apparently human hypertetraploid karyotype |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
