D283 Med（人脑髓母细胞瘤）
CBP60778
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| I. General information | |
| Synonyms: | D283 Med |
| Background: | The D283 Med cell line was established in 1985 by Friedman et al. from malignant ascites cells and a peritoneal metastasis from a boy with medulloblastoma. Derived from metastatic site, peritoneum |
| Species: | Homo sapiens, human |
| Tissue: | brain/cerebellum; derived from metastatic site: peritoneum |
| Disease: | medulloblastoma |
| Gender: | male, 6 years juvenile, Caucasian |
| Morphology: | epithelial |
| Growth Mode: | suspension, multicell aggregates and some adherent cells |
| Doubling Time: | 52 hrs |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 9,12 D13S317: 8,10 D16S539: 11 D5S818: 11 D7S820: 10 THO1: 7 TPOX: 8,11 vWA: 16,18 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) D283 Med完全培养基，# CBP60778M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Genes Expressed: | The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity. |
| Cellular Products: | neurofilament proteins (NFP) |
| Tumor Formation: | Yes, in nude mice (The cells form serially transplantable intercranial and subcutaneous tumors.) Yes, in soft agar |
| Comments: | The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. For more information, please contact Cobioer (4008-750-250). |
