MFE-296（人子宫内膜腺癌细胞）
CBP60840
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| I. General information | |
| Synonyms: | MFE-296 |
| Background: | established from the primary tumor of a 68-year-old Caucasian woman with moderately differentiated endometrial adenocarcinoma (grade G2) in 1991; cells were described in the literature to express androgen receptors (androgen was reported to inhibit cell proliferation), but no estrogen receptors; cells were described in the literature to be tumorigenic in nude mice |
| Species: | human (Homo sapiens) |
| Tissue: | endometrium |
| Disease: | endometrium adenocarcinoma |
| Gender: | N/A |
| Morphology: | epithelial-like polymorphic cells growing adherently in monolayers |
| Growth Mode: | N/A |
| Doubling Time: | ca. 50-60 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) MFE-296完全培养基，# CBP60840M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin + |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of LDH, NP |
| Cytogenetics: | human diploid karyotype with 20% polyploidy - 46<2n>XX, no consistent abnormality detected - matches published karyotype |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/I I-, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
