MC3T3-E1（小鼠胚胎成颅骨成纤维细（可分化为成骨））
CBP60946
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| I. General information | |
| Synonyms: | MC3T3-E1 |
| Background: | established from the calvaria of an embryo/fetus C57BL/6 mouse; described in the literature to differentiate to osteoblasts and to produce collagen |
| Species: | murine (mouse) (Mus musculus) |
| Tissue: | embryo/fetus |
| Disease: | embryo/fetus calvaria fibroblasts |
| Gender: | N/A |
| Morphology: | adherent fibroblast-like cells growing as monolayer |
| Growth Mode: | N/A |
| Doubling Time: | ca. 1-2 days |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
80% MEM α, nucleosides (GIBCO 12571063) + 20% FBS + 2 mM L-glutamine+ 1 mM sodium pyruvate MC3T3-E1完全培养基，# CBP60946M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | N/A |
| Fingerprint: | N/A |
| Species: | originally confirmed as mouse with IEF of AST, MDH, NP and recently as mus musculus by COI DNA Barcoding |
| Cytogenetics: | murine hypotetraploid karyotype with 11.5% polyploidy - 72(68-80)<4n> - centric fusion absent |
| Virus | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
