B82（小鼠皮肤成纤维细胞（对BrdU耐药））
CBP60977
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| I. General information | |
| Synonyms: | B82 |
| Background: | Derivative of NCTC clone 929 (ECACC catalogue no. 88102702) obtained by progressive selection in BUdR, has a cloning efficiency of 70-80%. |
| Species: | Mouse |
| Tissue: | adipose |
| Disease: | Mouse C3H/An sc areolar and adipose tissue |
| Gender: | N/A |
| Morphology: | Fibroblast |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
MEM+10%FBS+1%NEAA+1%NaP B82完全培养基，# CBP60977M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | 2n = 51 |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
