H4-II-E（大鼠肝癌上皮细胞）
CBP60983
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| I. General information | |
| Synonyms: | H4-II-E |
| Background: | From H-35 Reuber hepatoma. Tyrosine aminotransferase positive. Synthesises several serum proteins including albumin, transferrin, prothrombin. The presence of type C virus has been shown and can readily be verified by electron microscopy. |
| Species: | Rat |
| Tissue: | liver |
| Disease: | Rat liver hepatoma |
| Gender: | N/A |
| Morphology: | Epithelial |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
MEM+10%FBS+1%NEAA+1%NaP H4-II-E完全培养基，# CBP60983M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Not specified |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
