SYF（小鼠胚胎成纤维细胞（SV40））
CBP60992
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| I. General information | |
| Synonyms: | SYF |
| Background: | SYF (deficient for Src, Yes, and Fyn) cells were generated in 1997 from mouse embryos harboring functional null mutations in both alleles of the Src family protein tyrosine kinases, Src, Yes, and Fyn. The cells were immortalized before passage 2 by infection with a retroviral vector transducing the SV40 large T antigen. SYF cells also carry a gene for neomycin resistance. |
| Species: | Mus musculus, mouse |
| Tissue: | embryo |
| Gender: | 9 days gestation |
| Morphology: | fibroblast |
| Growth Mode: | adherent |
| Culture Medium: |
DMEM+10%FBS SYF完全培养基，# CBP60992M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Oncogene: | src -; yes -; fyn - |
| Comments: | Src family kinases have been implicated as key regulators of ligand-induced cellular responses including proliferation, survival, adhesion and migration. This is the first cell line isolated that lacks all three ubiquitously expressed Src family members. SYF cells display reduced mobility in wound healing and haptotaxis assays but not in platelet derived growth factor (PDGF) induced chemotaxis. For more information, please contact Cobioer (4008-750-250). |
