32D（小鼠IL-3依赖性细胞）
CBP60995
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| I. General information | |
| Synonyms: | 32D |
| Background: | established from long-term bone marrow cultures of C3H/HeJ mice infected with the Friend murine leukemia virus; cells are constitutively growth factor-dependent |
| Species: | murine (mouse) (Mus musculus) |
| Tissue: | bone |
| Disease: | bone marrow |
| Gender: | N/A |
| Morphology: | mostly round cells growing singly or in small clusters in suspension, some cells are loosely adherent; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 15 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
IMDM + 10% FBS + 10% vol conditioned medium of cell line WEHI-3 OR +100ng/ml IL-3(R&D) 32D完全培养基，# CBP60995M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD11b +, F4/80 (+); |
| Fingerprint: | N/A |
| Species: | originally confirmed as mouse with IEF of LDH, NP and with species PCR and recently as mus musculus by COI DNA Barcoding |
| Cytogenetics: | murine hypodiploid karyotype - 35(34-35)<2n> - carries 5 centric fusion markers |
| Virus | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
