CAL-12T（人非小细胞肺癌细胞）
CBP60050
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| I. General information | |
| Synonyms: | CAL-12T |
| Background: | established from a male patient with a non-small cell lung carcinoma in 1981 |
| Species: | human (Homo sapiens) |
| Tissue: | lung |
| Disease: | non-small cell lung carcinoma |
| Gender: | N/A |
| Morphology: | adherent epithelial-like cells growing in monolayers; image; image |
| Growth Mode: | N/A |
| Doubling Time: | ca. 50 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM+10%FBS CAL-12T完全培养基，# CBP60050M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP +, neurofilament -, vimentin - |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, NP |
| Cytogenetics: | human hypotriploid karyotype with 12% polyploidy - 59(57-59)<3n>X, +1, -2, -4, -5, -6, -6, -7, -11, -12, -13, -16, -16, -18, +20, +4-5mar, der(1;18)(p10;q10), der(1)t(X;1)(?;p?21)t(1;?)(q1?1;?), der(1)(q12->q10::p10->pter), add(3)(p11), add(4)(p11), add(5)(p15), add(9)(q1?)x1-2, add(11)(q24), dup(11)(q13q24), del(12)(p12), i(14q), add(19)(q11), i(21q) |
| Virus | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
