SNU-475（人肝癌细胞）
CBP60218
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| I. General information | |
| Synonyms: | SNU-475 |
| Background: | SNU-475 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient prior to cytotoxic therapy. Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum. |
| Species: | Homo sapiens, human |
| Tissue: | liver |
| Disease: | grade II-IV/V, hepatocellular carcinoma |
| Gender: | male, 43 years, Asian |
| Morphology: | epithelial |
| Growth Mode: | adherent, monolayer of diffusely spreading cells |
| Doubling Time: | 66 hrs |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,11 D16S539: 12 D5S818: 10,13 D7S820: 7,12 THO1: 7,9 TPOX: 8,9 vWA: 14 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS SNU-475完全培养基，# CBP60218M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | Blood Type AB; Rh + |
| Comments: | Grossly, the original tumor was massive growth type. Histologically, it was predominantly compact and minor trabecular type. The cultured cells are multinucleated. Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed. For more information, please contact Cobioer (4008-750-250). |
