RT112/84（人膀胱上皮癌）
CBP60316
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| I. General information | |
| Synonyms: | RT112/84 |
| Background: | A slow growing tumour which does not form colonies in soft agar. Tumorigenic in nude mice, RT1112/84 cells possess a low plating efficiency requiring 400 cells/dish to form any colonies. |
| Species: | human (Homo sapiens) |
| Tissue: | bladder |
| Disease: | Human bladder carcinoma epithelial |
| Gender: | N/A |
| Morphology: | Epithelial |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X CSF1PO: 10,11 D13S317: 13,14 D16S539: 11,13 D5S818: 10,13 D7S820: 11,12 THO1: 7 TPOX: 8,11 vWA: 14,17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) RT112/84完全培养基，# CBP60316M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Not specified |
| Subculture Routine: | Split sub-confluent cultures (70-80%) seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Subculture weekly - cells are difficult to trypsinise, and should be kept sub-confluent. |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
