ATDC5 （小鼠成软骨细胞系）
CBP60425
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| I. General information | |
| Synonyms: | ATDC5 |
| Background: | The chondrogenic cell line ATDC5 has been derived from teratocarcinoma AT805. Cells showed a sequential transition of phenotype in vitro. They encompass stages from mesenchymal condensation to calcification. Bone morphogenetic protein-2 (BMP-2) stimulated the sequential progression of early- and late-phase differentiation. Activation of parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor resulted in inhibition of this differentiation capacity. |
| Species: | Mouse |
| Tissue: | Unknown |
| Disease: | Mouse 129 teratocarcinoma AT805 derived |
| Gender: | N/A |
| Morphology: | Epithelial-like |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM:Ham's F12+10%FBS ATDC5完全培养基，# CBP60425M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Not specified |
| Subculture Routine: | Split sub-confluent cultures (70-80%) i.e. seeding at 1-3 x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA;CO2; 37°C subculture every 2-3 days. |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
