Cal27（人舌头鳞癌细胞）
CBP60427
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| I. General information | |
| Synonyms: | Cal27 |
| Background: | Cal 27 was established in 1982 by J. Gioanni (Centre Antoine Lacassagne, Nice Cedex, France) from tissue taken prior to treatment from a 56 year old Caucasian male with a lesion of the middle of the tongue. |
| Species: | Homo sapiens, human |
| Tissue: | tongue |
| Disease: | squamous cell carcinoma |
| Gender: | male, 56 years, Caucasian |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | 35 hrs |
| DNA Profile: | Amelogenin: X CSF1PO: 10,12 D13S317: 10,11 D16S539: 11,12 D5S818: 11,12 D7S820: 10 THO1: 6,9.3 TPOX: 8 vWA: 14,17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM+10%FBS Cal27完全培养基，# CBP60427M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | N/A |
| Receptor Expression: | N/A |
| Oncogene: | N/A |
| Genes Expressed: | N/A |
| Cellular Products: | N/A |
| Tumor Formation: | Yes, solid tumors developed within 6 weeks in nude mice inoculated with 2 x 106 cells subcutaneously |
| Comments: | CAL 27 cells are epithelial, polygonal with a highly granular cytoplasm. Immunocytochemical studies show strong positive staining with anti keratin antibodies. The cells do not grow well in semi-solid medium. Marked inhibition of thymidine incorporation was observed in the presence of VP16 (etoposide), CCNU (1-[2-chloroethyl]-3-cyclohexyl-1-nitrosourea), VM26 (teniposide), ADM (adriamycin), CPA (cyclophosphamide), and MTX (methotrexate). CAL 27 cells were resistant to treatment with VDS (vindesine sulfate), CDP (cis-platinum) or ACTD (actinomycin D). A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. For more information, please contact Cobioer (4008-750-250). |
