SCC-4（人舌头鳞癌细胞）
CBP60688
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| I. General information | |
| Synonyms: | SCC-4 |
| Species: | Homo sapiens, human |
| Tissue: | tongue |
| Disease: | squamous cell carcinoma |
| Gender: | male,55 years |
| Morphology: | epithelial-like |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 11 D13S317: 11,13 D16S539: 12 D5S818: 13 D7S820: 9,11 THO1: 9.3 TPOX: 8 vWA: 15,17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM/F12(1:1)+10%FBS+0.5 mM sodium pyruvate +400 ng/ml hydrocortisone SCC-4完全培养基，# CBP60688M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Genes Expressed: | epidermal keratins; 40 kD keratin |
| Cellular Products: | epidermal keratins; 40 kD keratin |
| Tumor Formation: | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Comments: | SCC-4 forms colonies in semi-solid medium, and is not induced to differentiate by anchorage deprivation.Clonal growth of these cells is improved by using a 3T3 (ATCC CCL-92) feeder layer (see Rheinwald and Green, Cell 6:331, 1975 for methods) Ref. ATCC grows these cells on 56-X.2, MITC-STO cells. The feeder cells must be plated 24 hours before use at 2 X 106/T75 in order to obtain a 30% confluent monolayer. For more information, please contact Cobioer (4008-750-250). |
