CMK（人原始巨核细胞白血病细胞）
CBP60531
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| I. General information | |
| Synonyms: | CMK |
| Background: | established from the peripheral blood of a 10-month-old boy with Down's syndrome and acute megakaryocytic leukemia (AML M7) at relapse in 1985 |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | acute megakaryocytic leukemia |
| Gender: | N/A |
| Morphology: | single, multiformed, large cells growing in suspension, sometimes loosely adherent (<3%); 2-3% giant, multinucleated cells; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 40-50 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+20%FBS CMK完全培养基，# CBP60531M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD41 +, CD71 +, CD235a +; image |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, NP |
| Cytogenetics: | human flat-moded hypotetraploid karyotype with 8% polyploidy - 85-90<4n>XY, -X, -Y, -2, -3, +5, -6, -6, -8, +11, -15, -15, +16, -17, -19, +21, +22, +7-11mar, add(1)(q31), add(1)(p36), add(3)(q11), del(3)(p14)x2-3, add(5)(q11), add(5)(q13), dup(8)(q11q21), add(8)(q13-21), del(8)(q11), del(9)(p21)x2, add(9)(q11)x2, del(10)(q22q24), der(11;17)(q10;q10), der(11)dup(11)(p13p15)t(5;11)(q11;p15)x1-2, del(11)(q23), add(12)(p13)x2, add(17)(p1?), add(18)(q23)x2-3, add(19)(p13), der(20)t(1;20)(q2?5;q1?2)x2, add(22)(q13) - disomic rearrangement of 20q1 close to common deleted segment, together with additional rearrangements of ch 20 present in markers - matches published karyotype |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
