DB（人B细胞淋巴瘤）
CBP60632
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| I. General information | |
| Synonyms: | DB |
| Background: | The DB cell line was established by Walter J. Urba and Dan L. Longo from ascites fluid from a patient with a diffuse large cell lymphoma. |
| Species: | Homo sapiens, human |
| Tissue: | Ascites |
| Disease: | large cell lymphoma |
| Gender: | Caucasian,male,45 years |
| Morphology: | lymphoblast |
| Growth Mode: | suspension |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 11,12 D16S539: 11 D5S818: 11 D7S820: 11 THO1: 6,8 TPOX: 8 vWA: 15,16 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS DB完全培养基，# CBP60632M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | CD19 +; CD20 +; CD22 +; Hle-1 +; CD21 -; CD25 - |
| Cellular Products: | immunoglobulin |
| Comments: | The cells do not express HLA-DR, HLA-DQ, IgM , IgD or T cell rantigen receptor. Exposure of DB cells to phorbol esters (PMA or PdBu) that activate protein kinase C results in profound growth inhibition. They are reported to be negative for the Epstein-Barr virus genome.The cells were cured of a prior mycoplasma contamination by a 21 day treatment with BM-cycline. For more information, please contact Cobioer (4008-750-250). |
