B95-8（EBV阳性棉顶狨猴淋巴细胞）
CBP61105
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| I. General information | |
| Synonyms: | B95-8 |
| Background: | established from peripheral blood lymphocytes of a cotton-top tamarin (cotton-top marmoset) monkey (Saguinus oedipus); cells were described in the literature to release high titers of Epstein-Barr virus (EBV) providing a source for transformation of B cells and establishment of continuous cell lines |
| Species: | monkey - cotton-top tamarin (Saguinus oedipus) |
| Tissue: | N/A |
| Disease: | lymphocytes |
| Gender: | N/A |
| Morphology: | single, round to oval cells or clustered in small clumps in suspension and partially adherent; ca. 10% adhere, but trypsin is not necessary to detach the cells; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 41 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS B95-8完全培养基，# CBP61105M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | N/A |
| Fingerprint: | unique Hinf I-(gtg)5 DNA profile |
| Species: | Sanguinus oedipus origin confirmed by COI DNA barcoding |
| Cytogenetics: | near-diploid simian karyotype with 4% polyploidy - 44(42-47)<2n> - chromosome rearrangements present |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
