Farage（人非霍奇金淋巴瘤细胞）
CBP60668
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| I. General information | |
| Synonyms: | Farage |
| Background: | The Farage cell line was adapted to culture in 1990 from a lymph node biopsy of a patient with diffuse large cell non-Hodgkin's lymphoma (DLCL). |
| Species: | Homo sapiens, human |
| Tissue: | derived from metastatic site: lymph node |
| Disease: | non-Hodgkin's B cell lymphoma |
| Gender: | White, female, adult |
| Morphology: | lymphoblast |
| Growth Mode: | suspension |
| Doubling Time: | 24 to 36 hrs |
| DNA Profile: | Amelogenin: X CSF1PO: 11,12 D13S317: 11,13 D16S539: 11,12 D5S818: 12 D7S820: 12 THO1: 8,9 TPOX: 9 vWA: 14,15 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS+0.05 mM 2-mercaptoethanol Farage完全培养基，# CBP60668M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | CD10 +/-; CD11a + (LFA-1); CD19 +; CD20 +; CD21 +; CD22 +; CD23 +; CD29 (VLA-4) +; CD38 +; CD39 +; CD40 +; CD44 +; CD54 + (ICAM-1); CD58 + (LFA-3); CD23 -; HLA DR + |
| Comments: | The cells do not express surface or cytoplasmic immunoglobulin.Exposure of Farage cells to phorbol 12-myristate 13-acetate (PMA) down-regulated CD21 and CD23 expression.hey do not express the terminal deoxynucleotydyl transferase gene (TdT), nor the recombination activating genes RAG-1 and RAG-2, known as markers of the pre-B cell stage. These results show that Farage represents a mature B-cell rather than a pre-B cell. Exposure to IL-4 augmented the concentrations of CD23, CD54, and CD58 but diminished the expression of CD21, CD22, and CD38. Incubation with IL-4 for 6 to 8 days led to increased expression of CD11a, CD39, CD40, and to disappearance of CD21 and CD38.The cells are positive for Epstein-Barr virus (EBV). mature B cel For more information, please contact Cobioer (4008-750-250). |
