MC3T3-E1 Subclone 14（小鼠颅顶前骨细胞亚克隆14）
CBP60868
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| I. General information | |
| Synonyms: | MC3T3-E1 Subclone 14 |
| Background: | A series of subclones were isolated from the cloned but phenotypically heterogeneous MC3T3-E1 cell line. |
| Species: | Mus musculus, mouse |
| Tissue: | bone, calvaria |
| Gender: | newborn |
| Morphology: | fibroblast |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12 D16S539: 9,11 D5S818: 12,13 D7S820: 8,12 THO1: 7 TPOX: 9,11 vWA: 17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
80% MEM α, nucleosides (GIBCO 12571063) + 20% FBS + 2 mM L-glutamine+ 1 mM sodium pyruvate MC3T3-E1 Subclone 14完全培养基，# CBP60868M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Genes Expressed: | collagen Ref |
| Cellular Products: | collagen Ref |
| Tumor Formation: | Yes, in immunodeficient mice (forms bone-like ossicles) |
| Comments: | The subclones were selected for high or low osteoblast differentiation and mineralization after growth in medium containing ascorbic acid. The MC3T3-E1 Subclone 4 (ATCC CRL-2593) and the MC3T3 Subclone 14 (ATCC CRL-2594) lines exhibit high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. They form a well mineralized extracellular matrix (ECM) after 10 days. The MC3T3 Subclone 24 (ATCC CRL-2595) and the MC3T3 Subclone 30 (ATCC CRL-2596) lines exhibit poor osteoblast differentiation after growth in ascorbic acid. They do not form ECM. They can be used as negative controls for Subclones 4 and 14.Mineralizing subclones selectively express mRNAs for the osteoblast markers, bone sialoprotein (BSP), osteocalcin (OCN), and the parathyroid hormone (PTH)/parathyroid hormone-related protein (PTHrP) receptor. Subclones with both high and low differentiation potential produce similar amounts of collagen in culture and express comparable basal levels of mRNA encoding Osf2/Cbfa1, an osteoblast-related transcription factor. After implantation into immunodeficient mice, highly differentiating subclones form bone-like ossicles resembling woven bone, while poorly differentiating cells only produce fibrous tissue. For more information, please contact Cobioer (4008-750-250) |
