RSC96（大鼠神经雪旺细胞）
CBP60873
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| I. General information | |
| Synonyms: | RSC96 |
| Background: | The RSC96 cell line is a spontaneously transformed rat Schwann cell line derived from long-term culture of rat primary Schwann cells. Ref |
| Species: | Rattus norvegicus, rat |
| Morphology: | neuronal |
| Growth Mode: | adherent |
| Culture Medium: |
DMEM+10%FBS RSC96完全培养基，# CBP60873M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Receptor Expression: | Nerve growth factor receptor (Ngfr); Protein and mRNA, negative Ref Platelet derived growth factor receptor, alpha; negative Ref Platelet derived growth factor receptor, beta; negative Ref |
| Oncogene: | Eerb-B2, positive [PubMed: 9766530]Erb-B3, negative [PubMed: 9766530]Products:cyclic nucleotide phosphodiesterase 1 (Cnp1) (CNPase); positive[PubMed: 9766530]laminin; positive [PubMed: 9766530]Myelin-associated glycoprotein (Mag); Protein and mRNA, negative [PubMed: 12210843] Myelin basic protein (Mbp); Protein and mRNA, negative [PubMed: 12210843]Myelin protein zero (Mpz) (Charcot-Marie-Tooth neuropathy 1B); Protein and mRNA, negative [PubMed: 12210843]peripheral myelin protein 22 (Pmp22); mRNA, positive; protein, negative [PubMed: 12210843]S100 calcium-binding protein, beta (neural) (S100b) protein, positive [PubMed: 12210843]Receptors expressed:Nerve growth factor receptor (Ngfr); Protein and mRNA, negative [PubMed: 12210843]platelet derived growth factor receptor, alpha; negative [PubMed: 9766530]platelet derived growth factor receptor, beta; negative [PubMed: 9766530] |
| Cellular Products: | cyclic nucleotide phosphodiesterase 1 (Cnp1) (CNPase); positive laminin; positive Ref Myelin-associated glycoprotein (Mag); Protein and mRNA, negative. Myelin basic protein (Mbp); Protein and mRNA, negative. Myelin protein zero (Mpz) (Charcot-Marie-Tooth neuropathy 1B); Protein and mRNA, negative. Peripheral myelin protein 22 (Pmp22); mRNA, positive; protein, negative. S100 calcium-binding protein, beta (neural) (S100b) protein; positive Ref |
| Comments: | A culture submitted to the ATCC in December of 2002 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative. For more information, please contact Cobioer (4008-750-250). |
