143BTK-（人骨肉瘤细胞）
CBP61118
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| I. General information | |
| Synonyms: | 143BTK- |
| Animal | human |
| Sex | Unknown |
| Tissue | osteosarcoma |
| Exogene | v-Ki-ras |
| Morphology | fibroblast-like |
| Anchoraged | Yes |
| Medium |
MEM+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antibiotics | Free |
| Growth temp | 37oC |
| Passage method | 0.25% trypsin |
| Split ratio | 1:8 split |
| Subculture frequency | twice/week |
| Cloned | No |
| Lifespan | infinite |
| Mycoplasma | - |
| Isozyme analysis | LD, NP |
| Chr distrb | 46-125(50):/48(11),49(13),50(10),51(4),53(2)/ |
| Short Tandem Repeat | OK |
| Comment | v-Ki-ras transformant TE85 cell line. HAT sensitive, neo resistant. |
