1.1B4（杂交细胞系1.1B4通过用人胰腺癌细胞系PANC-1电融合人胰岛的原代培养物而形成。）
CBP61117
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| I. General information | |
| Synonyms: | 1.1B4 |
| Background: | The hybrid cell line1.1B4 was formed by the electrofusion of a primary culture of human pancreatic islets with PANC-1, a human pancreatic ductal carcinoma cell line (ECACC catalogue number 87092802). According to the literature (Vasu et al 2013) (Green et al 2015) 1.1B4 secretes a low level of insulin as detected by radioimmunoassay (see protocol below). 1.1B4 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology The STR profile of 1.1B4 is indistinguishable from the parental PANC-1 Cell Line indicating that the DNA from the pancreatic beta cell fusion partner is below the limit of detection of the STR profiling assay. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis. |
| Species: | human (Homo sapiens) |
| Tissue: | Pancreatic islets |
| Disease: | A human pancreatic Beta cell: PANC-1 hybrid cell line. Insulin-secreting cell line. |
| Gender: | N/A |
| Morphology: | Epithelial |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 11 D5S818: 11,13 D7S820: 8 THO1: 7,8 TPOX: 8,11 vWA: 15 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS+5μg/ml Hydrocortisone + 10μg/ml Insulin 1.1B4完全培养基，# CBP61117M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Modal chromosome number 67-71 |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4 x 104 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 20hrs. At confluence 105 cells/cm² can be expected. |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
