CGM1（人EB病毒转化的B细胞）
CBP61129
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| I. General information | |
| Synonyms: | CGM1 |
| Background: | An Epstein-Barr virus (EBV) transformed lymphoblastoid cell line with Human Leukocyte Antigen (HLA)-typing data available. |
| Species: | human (Homo sapiens) |
| Tissue: | Blood |
| Disease: | Human HLA-Typed Lymphoblastoid Cell Line |
| Gender: | N/A |
| Hazard Group (ACDP): | 1 |
| Genetic Modification: | This is an Epstein-Barr Virus (EBV) transformed cell line |
| GMO Status: | Genetically Modified Organism Class 1 (GMO1) |
| Homozygous: | N |
| WS Region: | USA |
| Lab Code: | PAR |
| CellType: | Lymphoblastoid |
| Morphology: | Spherical |
| Growth Mode: | Suspension |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X CSF1PO: 11 D13S317: 9,13 D16S539: 14 D5S818: 11,12 D7S820: 11,13 THO1: 8,9.3 TPOX: 8,9 vWA: 14,17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS CGM1完全培养基，# CBP61129M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | N/A |
| Subculture Routine: | When resuscitating from frozen, cells should be seeded at 0.5 x 106 cells/ml in the culture medium. The culture should establish log phase growth after 3-5 days. When growing well dilute every 1-3 days to 3 x 105 cells/ml. Maintain cultures between 3-9 x 105 cells/ml for maximum growth; 5% CO2; 37°C. Cells form floating aggregates of varying size. Protocol: resuscitation of frozen cells |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
