C75（人Dukes'D期的中度分化的人结直肠腺癌）
CBP61183
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| I. General information | |
| Synonyms: | C75 |
| Background: | The C75 cell line was established from a 56-year old male patient with a moderately well differentiated adenocarcnoma of the sigmoid colon classified as Dukes' stage D |
| Species: | human (Homo sapiens) |
| Tissue: | Colon |
| Disease: | Human colorectal adenocarcinoma, moderately well differentiated, Dukes' stage D |
| Gender: | a 56-year old male |
| Morphology: | Epithelial |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 12 D13S317: 10,13 D16S539: 8,11 D5S818: 12 D7S820: 9,11 THO1: 8,9.3 TPOX: 10 vWA: 17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
IMDM + 10% FBS C75完全培养基，# CBP61183M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | N/A |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. C75 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells. |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
