Nthy-ori3-1（人类甲状腺滤泡上皮正常细胞）
CBP61205
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| I. General information | |
| Synonyms: | Nthy-ori3-1 |
| Background: | Normal human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SV-ori) to immortalise them. SV40 DNA was able to immortalise normal thyroid epithelial cells with the retention of several different functions. |
| Species: | human (Homo sapiens) |
| Tissue: | Thyroid |
| Disease: | Human thyroid follicular epithelial |
| Gender: | N/A |
| Morphology: | Epithelial |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X CSF1PO: 12 D13S317: 11 D16S539: 12,13 D5S818: 11 D7S820: 7,12 THO1: 7 TPOX: 9 vWA: 16,18 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI1640+10%FBS Nthy-ori3-1完全培养基，# CBP61205M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Not specified |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
