NCI-H322（白种人的细支气管肺泡癌）
CBP60134
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| I. General information | |
| Synonyms: | NCI-H322 |
| Background: | NCI-H322 was derived in 1981 from a primary bronchioalveolar carcinoma of the lung from a 52 year old male taken prior to treatment. Ultrastructural studies of this non-small cell carcinoma cell line demonstrated the presence of cytoplasmic structures characteristic of Clara cells. NCI-H322 do not express UDP-glucuronosyltransferase, but are reported to express glutathione-S-transferase and phenol sulfotransferase. Expression of protein and RNA of SP-A, the major surfactant-associated protein was detected. The cells produce tumours in athymic mice. |
| Species: | human (Homo sapiens) |
| Tissue: | Lung (cervical node metastasis) |
| Disease: | Human Caucasian bronchioalveolar carcinoma |
| Gender: | a 52 year old male |
| Morphology: | N/A |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 12 D13S317: 12 D16S539: 11,12 D5S818: 11 D7S820: 11 THO1: 7 TPOX: 8 vWA: 17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS NCI-H322完全培养基，# CBP60134M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Not specified |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
