NCI-H460（人大细胞肺癌）
CBP60138
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| I. General information | |
| Synonyms: | NCI-H460 |
| Background: | The NCI-H460 cell line was derived by A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with large cell cancer of the lung. |
| Species: | Homo sapiens, human |
| Tissue: | lung: pleural effusion |
| Disease: | carcinoma; large cell lung cancer |
| Gender: | male |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | 23 hrs in medium with serum; 42 to 60 hrs in serum |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 13 D16S539: 9 D5S818: 9,10 D7S820: 9,12 THO1: 9.3 TPOX: 8 vWA: 17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS NCI-H460完全培养基，# CBP60138M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Tumor Formation: | Yes, in nude mice |
| Comments: | The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein. For more information, please contact Cobioer (4008-750-250). |
