NCI-H596（人肺癌腺癌细胞）
CBP60142
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| I. General information | |
| Synonyms: | NCI-H596 |
| Background: | The NCI-H596 cell line was derived by A.F. Gazdar and H. Oie in 1983 from a tumor mass in the chest wall of a patient with adenosquamous carcinoma of the lung. The specimen was obtained prior to therapy. |
| Species: | Homo sapiens, human |
| Tissue: | lung |
| Disease: | adenosquamous carcinoma |
| Gender: | male, 73 years, Caucasian |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X CSF1PO: 12,13 D13S317: 11 D16S539: 11 D5S818: 11,13 D7S820: 11 THO1: 7,9.3 TPOX: 8 vWA: 16 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS NCI-H596完全培养基，# CBP60142M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Tumor Formation: | Yes, in nude mice |
| Comments: | The cells express easily detectable levels of p53 mRNA (comparable to levels found in normal lung), and exhibit no gross structural DNA abnormalities. The cells stain positively for keratin and vimentin, but are negative for neurofilament triplet protein. The cells express multiple markers of squamous differentiation, including cross linked envelope formation, transglutaminase activity and production of involucrin. For more information, please contact Cobioer (4008-750-250). |
