Ultra Luciferase Detection Kit
CBPH0001
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| Ultra Luciferase Detection Kit | ||||
| Descripition |
Ultra luciferase detection kit provides an assay reagent that generates ultra-bright signal in the presence of firefly luciferase. The assay reagent is prepared by mixing substrate and cell lysis buffer
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| 产品组分 | ||||
| 货号 | CBPH0001-1 | CBPH0001-2 | CBPH0001-3 | CBPH0001-4 |
| 规格 | 100 points | 1000 points | 5000 points | 10000 points |
| 组分A: Cell lysis buffer |
1 bottle 10 mL/bottle |
2 bottles 50 mL/bottle |
5×CBPH0001-2-A | 10×CBPH0001-2-A |
| 组分B: Substrate 50X |
1 vial 200μL/vial |
2 vials 1 mL/vial |
5×CBPH0001-2-B | 10×CBPH0001-2-B |
| 数据展示 | ||||
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1.Cobioer-UItra VS Bright-glo
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Figure 2. TNF α Dose Response Curve.
Figure 3. NF-KB Hek293 cells. |
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2.冻融测试
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Figure 4. TNF α Dose Response Curve. |
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3.定量和裂解能力测试
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Figure 5. Ultra vs Bright-glo. |
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| Protocol | ||||
| 1.检测试剂的准备 | ||||
| 使用前底物和细胞裂解液在4 ℃或室温下溶解。开盖前建议离心，集中液体到瓶底。按照1:50的体积比将组分B加入到组分A中，充分混匀配置所需量的检测试剂。 | ||||
| 2.萤火虫萤光素酶的检测 | ||||
| ·检测试剂和测试细胞样本平衡至室温。 | ||||
| ·向测试细胞样本中加入等体积的检测试剂。 | ||||
| 96孔板检测体系，100μL培养基中加入100μL检测试剂。 | ||||
| ·在测量前1200转震荡5分钟，充分震荡会使效果更好。 | ||||
| 3. Notes | ||||
| ·不同批号试剂盒中的试剂不可混合使用。 | ||||
| ·不透明板子用于检测，白色不透明的96孔检测效果更好。 | ||||
| Product Information | ||||
| Intended Use | Research Use Only | |||
| Unit Size | 100 points/1000 points/5000 points/10000 points | |||
| Storage | -80℃~-20℃ | |||
| Expiry | 12 months from the date of manufacture | |||
