C5aR1/CHO
CBP71374
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| I. Background | |
| C5AR1 (Complement C5a Receptor 1) is a Protein Coding gene. Diseases associated with C5AR1 include Hypersensitivity Reaction Type Iii Disease and Mast-Cell Sarcoma. Among its related pathways are Complement cascade and GPCR downstream signalling. | |
| II. Introduction | |
| Host Cell: |
CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5ug/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for C5aR1 receptor |
| Transducer: | Gi |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| Ⅳ. Representative Data | |
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Figure 1. Recombinant C5aR1/CHO constitutively expressing C5aR1. |
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Figure 2. HTRF cAMP Assay With C5aR1 CHO Cells (C7). |
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