CCR5/Gα15/CHO
CBP71057
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| I. Background | |
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CCR5（趋化因子受体 5 或趋化因子 C－C 亚族受体 5），也称为 CD195，是一种 G蛋白 偶联受体，属于整合型膜蛋白的 β 趋化因子受体家族成员。CCR5 主要与 Gα i/o 和 Gα q/11 偶联，在树突状细胞、T 细胞、巨噬细胞、嗜酸性粒细胞、小胶质细胞和髓系抑制细胞上 表达，并由多种配体（CCL3、CCL3L1、CCL4、CCL5、CCL8、CCL11、CCL13 和 CCL16） 激活，以促进趋化性和免疫细胞活化。
巨噬细胞和树突状细胞分泌 CCR5 依赖性促炎细胞因子可激活适应性免疫反应。活化 的 CD4+ T 细胞分泌 CCR5 依赖性 IL-2 可诱导效应 T 细胞、记忆 T 细胞和调节性 T 细胞的 增殖和活化。 CCR5 是加速募集效应和记忆 CD8+ T 细胞至感染部位所必需的。CCR5 是 HIV 进入免疫细胞的辅助受体。CCR5 可与多种配体任意结合，从而启动包括引导迁移在 内的细胞信号传导。 |
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| II. Introduction | |
| Host Cell: |
CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
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Gene Synonyms: |
C-C chemokine receptor type 5, CCR5 |
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Expressed Gene: |
GenBank accession number NM_000579; no expressed tags |
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Host Cell: |
CHO-K1 |
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Stability: |
32 passages |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F-12+10%FBS+3μg/ml puromycin+100μg/ml Hygromycin B |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for CCR5 |
| Transducer: | Gi |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| Ⅳ. Representative Data | |
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Concentration-dependent stimulation of intracellular calcium mobilization by MIP-1α in CCR5/Gα16/CHO and Gα15/CHO cells Figure 1. MIP-1α-induced concentration-dependent stimulation of intracellular calcium mobilization in CCR5/Gα16/CHO and Gα15/CHO cells. The cells were loaded with Calcium-4 prior to stimulation with an CCR5 receptor agonist, MIP-1α. The intracellular calcium change was measured by FlexStation. |
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