DRD1/CHO
CBP71408
询 价
索取protocol
产品描述
产品数据库
| I. Background | |
| The D1 subtype of the dopamine receptor is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor is Gs/a coupled and indirectly activates cyclic AMP-dependent protein kinase, stimulating the neuron. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events.Alternative transcription initiation sites result in two transcript variants of the gene. D1-D2 dopamine receptor heteromer formation is observed. | |
| II. Introduction | |
| Host Cell: |
CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12k+10%FBS+5ug/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for DRD1 |
| Transducer: | Gs |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| Ⅳ. Representative Data | |
|
Figure 1. Recombinant DRD1/CHO constitutively expressing DRD1. |
|
|
Figure 2. Dopamine hydrochloride effect on CHO-DRD1 clone 37. |
|
