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BRAF V600K/BaF3

CBP73390

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I. Introduction
Cell Line Name: BRAF V600K/BaF3
Host Cell: Ba/F3
Stability: 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Application: Anti-proliferation assay and PD assay
Freeze Medium: 90% FBS+10% DMSO
Complete Culture Medium: RPMI-1640+10%FBS+2ug/ml puromycin
Mycoplasma Status: Negative
 
II. Background
BRAF, serine/threonine-protein kinase B-raf, is a member of the Raf family of serine/threonine protein kinases, which signals through the MAP kinase pathway to regulate cell proliferation and cell growth. 
 
III. Representative Data

1. WB of BRAF V600K/BaF3

2. Sanger of BRAF V600K/BaF3

3. Anti-proliferation assay

Figure 3. CTG Proliferation Assay of BRAF V600K BaF3 Cells (C3).

 

 

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