HCT116 MTAP-/-cell
CBP75002
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| I. Background | |
| MTAP (Methylthioadenosine Phosphorylase) is a Protein Coding gene. Diseases associated with MTAP include Diaphyseal Medullary Stenosis With Malignant Fibrous Histiocytoma and Bone Sarcoma. Among its related pathways are Cytokine Signaling in Immune system and Interleukin-12 family signaling. Gene Ontology (GO) annotations related to this gene include 1,4-alpha-oligoglucan phosphorylase activity and S-methyl-5-thioadenosine phosphorylase activity. | |
| II. Description | |
| HCT116 MTAP -/- cells have been homozygously knocked out of MTAP gene. | |
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III. Introduction |
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| Host Cell: | HCT116 |
| Knockout gene: |
MTAP，Homo sapiens (human) |
| Stability: | 32 passages |
| Synonym(s): | methylthioadenosine phosphorylase,BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | McCoy’s 5A+10%FBS |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Colon |
| Disease: | Colorectal Carcinoma |
| Morphology: | Epithelial-like small cells growing as monolayer |
| Growth Properties: | Adherent |
| V. Representative Data | |
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1. WB of HCT116 MTAP-/-cell
Figure 1. WB of MTAP expression. |
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2. Sanger of HCT116 MTAP-/-cell
Figure 2. Sanger sequencing of MTAP: p.M140Rfs*6. |
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3. Anti-proliferation assay
Figure 3. CTG Proliferation Assay of HCT116 MTAP KO cells.
Figure 4. CTG Proliferation Assay of HCT116 Parental cells. |
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