HEL（人红白血病细胞）
CBP60721
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| I. General information | |
| Synonyms: | HEL |
| Background: | established from the peripheral blood of a 30-year-old man with erythroleukemia (AML M6) in relapse (after treatment for Hodgkin lymphoma) in 1980; cells were described in the literature to be capable of spontaneous and induced globin synthesis and to carry the JAK2 V617F mutation |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | erythroleukemia |
| Gender: | N/A |
| Morphology: | round, large to occasionally giant polynucleated, single cells in suspension; few cells adherent; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 36 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays |
| Immunology: | CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, CD41a +, CD71 +, CD235a +; |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, MDH, NP |
| Cytogenetics: | human hypotriploid karyotype with 2.3% polyploidy - 63(60-64)<3n>XYY, -2, -9, -10, -10, -11, -14, -16, -16, -17, -19, +20, +21, +2mar, del(2)(q32), t(3;6)(p13;q16), der(5)t(5;17)(q10;q10), der(6)t(1;6)(p13;p21), der(7)add(7)(p14;q32), add(8)(p21), der(9)t(9;?)(?;11)(p24;?)(?;q13), del(11)(q13), add(15)(p11), del(20)(q13), r(20)(p11q11), dup(21)(q11q22.3-qter), psu dic(22;9)t(9;?)(?;22)(p24;?)(?;p11-13) - resembles published karyotype - carries also masked 5q- and 20q- consistent with AML M6/MDS |
| Molec. Genetics: | expression of mutated JAK2 confirmed by RT-PCR and sequencing |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
