UT-7（人类原巨核细胞白血病细胞）
CBP60809
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| I. General information | |
| Synonyms: | UT-7 |
| Background: | established from the bone marrow of a 64-year-old man with acute myeloid leukemia (AML M7) at diagnosis in 1988; cells are constitutively cytokine-dependent and responsive to various cytokines |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | acute myeloid leukemia |
| Gender: | N/A |
| Morphology: | round (relatively large) cells growing singly in suspension (ca. 1-2% cells are slightly adherent); |
| Growth Mode: | N/A |
| Doubling Time: | ca. 70 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS+3IU/ml EPO UT-7完全培养基，# CBP60809M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD4 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, cyCD68 +; |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of LDH, NP |
| Cytogenetics: | human flat-moded hypotetraploid karyotype - 72-88<4n>XXYY, +1, -8, -9, -9, -9, -11, -11, -12, -13, -13, -14, -18, -21, -22, +6mar, del(1)(q42)x2, t(2;4)(p16-23;q27-31)x1-2, der(2)t(2;5)(p16-23;q12-14)x1-2, add(5)(q11), del(5)(q13), der(6;13)(p10;q10), i(7q)x1-2, add(8)(q24)x1-3, del(9)(q22), add(12)(p12-13), der(13)t(13;13)(p11;q14), add(14)(p11), add(15)(p11), add(16)(p1?), add(17)(p11), add(19)(p13)x2, add(19)(q13), i(21q)x1-2 - extensive subclonal variation present - resembles published karyotype |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
