LS513（人盲肠癌细胞）

CBP61154

询价

索取STR

产品描述

产品数据库

I. General information
Synonyms:	LS513
Background:	LS513 is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy from a 63 year old Caucasian male patient diagnosed with a Dukes' C mucin secreting cecal tumor located at the Bauhin valve.
Species:	Homo sapiens, human
Tissue:	cecum
Disease:	Dukes' type C,colorectal carcinoma
Gender:	male， 63 years， Caucasian
Morphology:	epithelial
Growth Mode:	adherent
Doubling Time:	25 hrs
DNA Profile:	Amelogenin: X,Y CSF1PO: 10,13 D13S317: 9,10 D16S539: 12,13 D5S818: 9,11 D7S820: 8,11 THO1: 8 TPOX: 8 vWA: 16,17 Cobioer’s Cell Line Authentication Service
Culture Medium:	RPMI-1640+10%FBS LS513完全培养基，# CBP61154M We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium:	90%FBS+10%DMSO
Antigen Expression:	carcinoembryonic antigen (CEA); ICAM-1; HLA class I positive
Receptor Expression:	N/A
Karyotype	N/A
Virus Susceptibility	Vesicular stomatitis, Orsay (Indiana) Vesicular stomatitis, Glasgow (Indiana) Human poliovirus 1
Oncogene:	p53 + (wild type)
Genes Expressed:	transforming growth factor beta 1 (TGF beta-1, 83 pg per 106 cells per 24 hours)
Cellular Products:	transforming growth factor beta 1 (TGF beta-1, 83 pg per 10 exp6 cells per 24 hours)
Tumor Formation:	Yes, forms tumors in nude mice
Comments:	LS513 is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy from a 63 year old Caucasian male patient diagnosed with a Dukes' C mucin secreting cecal tumor located at the Bauhin valve. Approximately 50% of LS513 cells express surface carcinoembryonic antigen (CEA). LS513 cells express the major histocompatibility (MHC) class I antigens HLA and beta 2 microglobulin. MHC class II antigens (HLA-DR, DQ, and DP were not detected). TGF beta-1 is inhibitory for proliferation of LS513 cells, whereas TGF beta-2 has no effect on the growth of these cells. LS513 cells are 100-fold less sensitive to TGF beta-1 than the LS1034 (ATCC CRL-2158) cell line. LS513 cells are multidrug resistant (MDR) and are tumorigenic in nude mice. Colony forming efficiency was 30% in methylcellulose. For more information, please contact Cobioer (4008-750-250).