FOXP3 promoter-Luc/Jurkat
CBP74011
询 价
索取Protocol
产品描述
产品数据库
| I. Background | |
| Foxp3, belonging to the forkhead family, is a master transcription factor that expresses exclusively in regulatory T cells, a subset of CD4+ T cells. Regulation of Foxp3 is critical for maintaining immunological tolerance. Over-expression of Foxp3 is known to suppress effector T cell activation. | |
| II. Description | |
|
Human Foxp3 luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by a human Foxp3 promoter and an enhancer-like conserved noncoding sequence upstream of the Foxp3 promoter.
Figure 1. Illustration of Foxp3 promoter region with representative transcription factor binding sites and enhancer regions. |
|
| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: | Foxp3-Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | Foxp3, Foxp3 cell line, scurfin cell line, foxo |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1 ug/ml puromucin |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
|
IV. Description of Host Cell Line |
|
| Organism: | Homo sapiens, human |
| Tissue: | Peripheral blood |
| Disease: | Acute T cell leukemia |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
|
|
|
|
Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Foxp3 Jurkat Reporter Activity in response to PMA with lonomycin. |
|
