AHRE Luciferase Reporter HT29
CBPM0001
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| I. Background | |
| FICZ (6-Formylindolo[3,2-b]carbazole) is a highly potent ligand of the cytosolic aryl hydrocarbon receptor (AhR) transcription factor. The presence of FICZ-derived metabolites in human urine points FICZ to be an endogenous AhR agonist, but which requires diet-derived tryptophan (Trp) amino acid. |
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| II. Introduction | |
| Host Cell: | HT29 |
| Expressed gene: | FICZ-AHRE Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+100ug/ml hygromycin |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Colon |
| Disease: | Colorectal adenocarcinoma |
| Morphology: | Epitheloid cells growing as monolayers and in large colonies |
| Growth Properties: | Adherent |
| IV. Representative Data | |
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Figure 1. Dose Response of FICZ in AHRE-Luc HT29 Cells (C5)(6 Hours). |
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Figure 2. Dose Response of FICZ in AHRE-Luc HT29 Cells (C5) (24 Hours). |
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