CAL-120（人乳腺癌细胞）
CBP60358
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| I. General information | |
| Synonyms: | CAL-120 |
| Background: | established from the pleural effusion of a 43-year-old woman with metastatic breast adenocarcinoma in 1991 |
| Species: | human (Homo sapiens) |
| Tissue: | breast |
| Disease: | breast adenocarcinoma |
| Gender: | N/A |
| Morphology: | heterogenous population with epithelial-like and fibroblast-like adherent cells growing in monolayers |
| Growth Mode: | N/A |
| Doubling Time: | ca. 50-60 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM+10%FBS CAL-120完全培养基，# CBP60358M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin + |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human by cytogenetics |
| Cytogenetics: | human hypertetraploid karyotype with 20% polyploidy 98(88-98)<4n>XXX/XXXX, -1, -2, -2, -4, +5, +5, +6, -9, -9, +10, +11, -12, -12, -13, -13, -14, -15, -16, -16, -20, -21, -22, +16-18mar, + 1-2 dmin, add(X)(p2?2), add(1)(q22), del(3)(q22), add(3)(p1?4), del(4)(p15.2), add(6)(q11)x2, del(7)(q32), add(8)(p12), add(8)(q24), der(8;13)(q10;q10), ins(11;?)(q13;?), add(12)(p1?), add(13)(p11)x1-2, add(14)(p11), der(15;?21)(p1?1;?q12)x3 - sideline with i(1)(p10)add(1)(p3?2), i(1q), i(3p), der(16)t(5;16)(q13;q24) |
| Virus | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
