CAL-51（人乳腺癌细胞）

CBP60360

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I. General information
Synonyms:	CAL-51
Background:	established from the pleural effusion metastasis of a 45-year-old woman with progressive breast adenocarcinoma (after radio-, chemotherapy and surgery) in 1985; rare example of tumor cell line with normal karyotype
Species:	human (Homo sapiens)
Tissue:	breast
Disease:	breast carcinoma
Gender:	N/A
Morphology:	epithelial-like adherent cells growing in monolayers; the culture contains usually a large amount of cell debris;
Growth Mode:	N/A
Doubling Time:	50-60 hours
DNA Profile:	Amelogenin: X CSF1PO: 11,12 D3S1358: 16,17 D5S818: 12,13 D7S820: 7,12 D8S1179: 14 D13S317: 11,13 D16S539: 11,13 D18S51: 15,17 D21S11: 31,32 FGA: 22,23 Penta D: 10,14 Penta E: 7,12 TH01: 7 TPOX: 8,9 vWA:18 Cobioer’s Cell Line Authentication Service
Culture Medium:	DMEM+10%FBS CAL-51完全培养基，# CBP60360M We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium:	90%FBS+10%DMSO
Mycoplasma:	negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:	cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint:	multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:	confirmed as human with IEF of AST, LDH, NP
Cytogenetics:	human near-diploid karyotype with 28% tetraploidy - 46<2n>XX, no consistent abnormality detected - rare example of tumor cell line with normal karyotype - resembles published karyotype
Virus	ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments:	For more information, please contact Cobioer (4008-750-250).

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