EVSA-T（人乳腺癌细胞）

CBP60367

询价

索取STR

产品描述

产品数据库

I. General information
Synonyms:	EVSA-T
Background:	established from the malignant ascitic effusion from a woman with metastatic breast carcinoma; described as being estrogen receptor-negative and progesterone receptor-positive
Species:	human (Homo sapiens)
Tissue:	breast
Disease:	breast carcinoma
Gender:	N/A
Morphology:	adherent epitheloid cells growing in monolayers;
Growth Mode:	N/A
Doubling Time:	ca. 50 hours
DNA Profile:	N/A Cobioer’s Cell Line Authentication Service
Culture Medium:	MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) EVSA-T完全培养基，# CBP60367M We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium:	90%FBS+10%DMSO
Mycoplasma:	contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:	cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Fingerprint:	multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:	confirmed as human with IEF of AST, MDH, NP
Cytogenetics:	human hypotriploid karyotype with 4% polyploidy - 62(55-64)<3n>XXX, +1, -2, -5, -5, +6, -8, -9, -10, -13, -13, -14, -15, -16, -17, -18, -20, -22, +6-8mar, add(X)(p11), der(X)t(X;9)(q21;q11), add(1)(p1?1), der(1)del(1)(p36)dup(1)(q22q32), del(1)(q11), der(1;4)(q10;q10)del(4)(q1?13q?23), der(2)t(2;5)(p11;q13)add(2)(q3?5), der(3)t(3;5)(p14;q11), del(4)(q22), del(6)(q25q27), del(6)(p11), add(6)(p23), del(7)(p14), add(8)(q24), ider(8)(q10)ins(8;?)dup(8)(?q21q24)add(8)(q24), add(11)(p15), add(12)(p13), del(12)(p13), dup(13)(q21q33), add(21)(p11), add(21)(p11), large submetacentric der(8) hsr marker present
Virus	ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV -
Comments:	For more information, please contact Cobioer (4008-750-250).