MCF 10A（人正常乳腺上皮细胞）
CBP60419
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| I. General information | |
| Synonyms: | MCF 10A |
| Background: | MCF 10A was derived from adherent cells in the population. |
| Species: | Homo sapiens, human |
| Tissue: | mammary gland; breast |
| Disease: | fibrocystic disease |
| Gender: | female, female, Caucasian |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X CSF1PO: 10,12 D13S317: 8,9 D16S539: 11,12 D5S818: 10,13 D7S820: 10,11 THO1: 8,9.3 TPOX: 9,11 vWA: 15,17 Cobioer’s Cell Line Authentication Service |
| Isoenzymes: | AK-1, 1 Ref ES-D, 1 Ref G6PD, B Ref GLO-I, 1-2 Ref PGM1, 1-2 Ref PGM3, 1 Ref |
| Culture Medium: |
MEGM+100 ng/ml cholera toxin MCF 10A完全培养基，# CBP60419M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | N/A |
| Receptor Expression: | N/A |
| Oncogene: | N/A |
| Genes Expressed: | N/A |
| Cellular Products: | N/A |
| Tumor Formation: | No, in immunosuppressed mice Ref Yes, in semisolid medium Ref |
| Comments: | The MCF 10A cell line is a non-tumorigenic epithelial cell line. The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. They exhibit three dimensional growth in collagen, and form domes in confluent cultures. Thus far, the cells have shown no signs of terminal differentiation or senescence. The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF). By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells. They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies. The calcium content of the medium exerts a strong effect on the morphology of the cells. For more information, please contact Cobioer (4008-750-250). |
