Panc 04.03（人胰腺腺癌细胞）
CBP60683
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| I. General information | |
| Synonyms: | Panc 04.03 |
| Background: | Panc 04.03 is a pancreatic adenocarcinoma epithelial cell line derived, in 1995, from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma. |
| Species: | Homo sapiens, human |
| Tissue: | pancreas |
| Disease: | adenocarcinoma |
| Gender: | White, male, 70 years adult |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | 20 hrs |
| DNA Profile: | Amelogenin: X CSF1PO: 12 D13S317: 11,12 D16S539: 11,12 D5S818: 11 D7S820: 8,12 THO1: 9.3 TPOX: 10,11 vWA: 14,16 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+15%FBS+10ug/ml insulin Panc 04.03完全培养基，# CBP60683M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | MHC class I +; MHC class II - ，Ref，Blood type O; Rh+ |
| Oncogene: | K-ras + Ref |
| Genes Expressed: | cytokeratins 7 and 18 Ref |
| Cellular Products: | cytokeratins 7 and 18 ref |
| Tumor Formation: | Yes, forms tumors in nude or SCID mice |
| Comments: | The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. Ref The cells have a reported plating efficiency of 80%. Ref For more information, please contact Cobioer (4008-750-250). |
