HPAF-II（人胰腺腺癌细胞）
CBP60836
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| I. General information | |
| Synonyms: | HPAF-II |
| Background: | HPAF-II is a human pancreatic adenocarcinoma cell line derived from peritoneal ascitic fluid of a 44 year old Caucasian male with primary pancreatic adenocarcinoma and metastases to the liver, diaphragm and lymph nodes. |
| Species: | Homo sapiens, human |
| Tissue: | pancreas |
| Disease: | adenocarcinoma |
| Gender: | male, 44 years, Caucasian |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X CSF1PO: 10,11 D13S317: 12 D16S539: 11,13 D5S818: 11,13 D7S820: 10,13 F13A01: 5,17 F13B: 8,10 FESFPS: 11,12 LPL: 10 THO1: 9 TPOX: 8 vWA: 17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
MEM+10%FBS+1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) HPAF-II完全培养基，# CBP60836M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | Blood Type A; Rh-; HLA A1, A10 (W34), B8, W22, Cw3 |
| Genes Expressed: | mucin |
| Cellular Products: | mucin |
| Tumor Formation: | Yes, the cells form well differentiated adenocarcinomas in athymic mice which resemble the original tumor |
| Comments: | HPAF II is a spontaneous variant with unlimited replicative capability which proliferated from a static culture of HPAF-I cells. The cells express Muc 1 and Muc 4 mucin genes, and secrete high levels of Muc 1 mucin. They are pleomorphic and probably undergo some spontaneous differentiation in culture For more information, please contact Cobioer (4008-750-250). |
