hTERT-HPNE（人胰腺导管细胞）
CBP60857
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| I. General information | |
| Synonyms: | hTERT-HPNE |
| Background: | The hTERT-HPNE cell line was developed from human pancreatic duct by transduction with a retroviral expression vector (pBABEpuro) containing the hTERT gene. The infected cells became positive for telomerase, failed to senesce, and were still proliferating after more than 150 doublings Ref. |
| Species: | Homo sapiens, human |
| Tissue: | Pancreas, duct |
| Disease: | Normal |
| Gender: | Male， 52 years |
| Morphology: | Epithelial-like |
| Growth Mode: | adherent |
| DNA Profile: | D5S818: 11 D13S317: 12,13 D7S820: 9,10 D16S539: 12,13 vWA: 17 Amelogenin: X,Y TPOX: 8,11 CSF1PO: 12 TH01: 8,9 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM+25%M3 medium+5%FBS+10ng/ml EGF hTERT-HPNE完全培养基，# CBP60857M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | Exposing hTERT-HPNE cells to sodium butyrate and 5-aza-2'-deoxycytidine lead to the formation of pancreatic ductal cells marked by the expression of p-glycoprotein (multidrug resistance (MDR-1)), carbonic anhydrase II, and the cytokeratins 7, 8, and 19. hTERT-HPNE cells were found to have properties of the intermediary cells formed during acinar-to-ductal metaplasia, which included their undifferentiated phenotype, expression of Nestin and, evidence of active Notch signaling Ref. For more information, please contact Cobioer (4008-750-250). |
