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TLR9/NFκB-Luc/HEK293

CBP74091

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I. Background

Recombinant HEK293 cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human TLR9 (Toll-like receptor 9, also been designated as CD289, a member of the toll-like receptor (TLR) family. GenBank Accession No. NM_017442).

 
II. Introduction
Host Cell: HEK293
Expressed gene: TLR9、NFκB-Luc
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): TLR, TLR-9, tlr, nfkb, nf-kb
Freeze Medium: 90% FBS+10% DMSO
Culture Medium:
MEM+10%FBS+1% non-essential amino acids(NEAA)+1 mM Na pyruvate+50 μg/ml  Hygromycin B+2ug/ml puromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
III. Description of Host Cell Line
Organism: Human
Tissue: Embryonic kidney
Disease: Normal
Morphology: Epitheloid cell
Growth Properties: Adherent
 
IV. Representative Data


Figure 1.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). TLR9/NF-κB Reporter - HEK293 Recombinant Cell Line (C3).

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